Double Strand DNA Fragments are Separated by Size Using

Question

Double strand DNA fragments are separated by size using
A) restriction analysis.
B) FISH.
C) PCR.
D) northern blotting.
E) gel electrophoresis.

Accepted Answer

Gel electrophoresis is the best choice for separating double strand DNA fragments by size. The DNA molecules are loaded into wells on a gel matrix, and an electric current is passed through the gel. The negatively charged DNA molecules are attracted to the positive electrode and move through the gel toward it. Smaller fragments move faster and further through the gel than larger fragments, creating distinct bands that can be visualized by staining.