Which of the Following Procedures Would Be Used to Introduce

Question

Which of the following procedures would be used to introduce DNA into a single mouse cell?
A) Southern blotting
B) electroporation
C) gene gun
D) microinjection
E) protoplast fusion

Accepted Answer

Microinjection is the process of injecting a foreign substance, such as DNA, directly into a cell using a very fine needle. It is a highly precise method that is often used for introducing DNA into a single cell, such as in the case of creating transgenic animals. Southern blotting is a technique used to detect specific sequences of DNA, electroporation is a method of introducing DNA into cells using an electric field, gene gun is a biolistic method of DNA delivery, and protoplast fusion is a technique used to combine two different cells by removing their cell walls.