Which Technique is Used to Select Novel Enzymes or Peptides

Question

Which technique is used to select novel enzymes or peptides produced through directed evolution?
A) mutagenesis with transposons
B) enzyme phage display
C) multiplex PCR
D) ethane methyl sulfonate mutagenesis
E) directional cloning

Accepted Answer

Enzyme phage display is a technique used to select novel enzymes or peptides produced through directed evolution. It involves displaying a library of enzymes or peptides on the surface of a bacteriophage and screening them for the desired activity or property. Mutagenesis with transposons, multiplex PCR, ethane methyl sulfonate mutagenesis, and directional cloning are other techniques used in genetic engineering, but they are not specifically used for selecting novel enzymes or peptides produced through directed evolution.