The Applications of the Yeast Two-Hybrid Method Include All of the Following

Question

The applications of the yeast two-hybrid method include all of the following EXCEPT
A) mapping protein-protein interactions in E. coli.
B) identifying of physiologically significant protein-protein interactions as potential antibiotic targets.
C) finding unknown proteins ("prey") that interact with proteins of known functions ("bait").
D) mapping protein-protein interactions in M. tuberculosis.
E) the ability to probe interactions in vivo.

Accepted Answer

The yeast two-hybrid method cannot be used to map protein-protein interactions in M. tuberculosis because it requires the use of a eukaryotic host, such as yeast or mammalian cells.