Advantages in Real-Time PCR Over Regular, End-Time PCR Include All

Question

Advantages in real-time PCR over regular, end-time PCR include all of the following EXCEPT
A) initial amounts of template DNA can be quantified by the length of time it takes to detect PCR products.
B) amplified DNA is quantified while the PCR is still taking place.
C) earlier increments in fluorescence indicate larger amounts of template DNA in the original sample.
D) real-time PCR uses GadX.
E) real-time PCR can be used to quantify DNA or RNA in a sample.

Accepted Answer

Real-time PCR does not use GadX (a hypothetical protein), so option D is the exception. The other options (A, B, C, and E) are advantages of real-time PCR over regular, end-time PCR.