Which Two Components of the Gram Stain Form a Complex

Question

Which two components of the Gram stain form a complex that is retained by Gram-positive cells?
A) crystal violet and iodine
B) safranin and iodine
C) crystal violet and safranin
D) alcohol and safranin
E) alcohol and iodine

Accepted Answer

Crystal violet and iodine form a complex that is retained by the thick peptidoglycan layer of Gram-positive cells, leading to their purple color after staining. Gram-negative cells, on the other hand, have a thinner peptidoglycan layer that is unable to retain this complex and are decolorized by the alcohol wash.