Fluorescent Microscopy That Absorbs Light at 260m Would MOST Likely

Question

Fluorescent microscopy that absorbs light at 260m would MOST likely fluoresce at
A) 100 nm.
B) 200 nm.
C) 260 nm.
D) 400 nm.
E) 800 nm.

Accepted Answer

Fluorescent microscopy works by absorbing light at one wavelength and then emitting light at a higher wavelength. This process is called fluorescence. The wavelength at which a fluorescent molecule absorbs light is called its excitation wavelength, while the wavelength at which it emits light is called its emission wavelength. In this case, since the fluorescent microscopy absorbs light at 260 nm, it is most likely to fluoresce at a higher wavelength, such as 400 nm. Therefore, the correct answer is choice D.