When the ribosome reaches a stop codon on the mRNA, no corresponding tRNA enters the A site. If the translation reaction were to be experimentally stopped at this point, which of the following would you be able to isolate?

A) an assembled ribosome with a polypeptide attached to the tRNA in the P site
B) separated ribosomal subunits, a polypeptide, and free tRNA
C) an assembled ribosome with a separated polypeptide
D) separated ribosomal subunits with a polypeptide attached to the tRNA
E) a cell with fewer ribosomes