You are interested in the regulation of gene Q.Proteins G, H, and J are proteins that are important for regulating gene Q, and bind to its promoter region in a sequence-specific fashion.Proteins G and H both bind to site "A" but cannot bind to site "A" at the same time.Protein J binds to site "B" on the promoter.The promoter region is diagrammed in Figure 8-20. experiment reaulator protein added Q mRNA number 12345678 G −+−−++−+H−−+−+−++J−−−+−+++ made? no no yes yes no yes yes yes Table 8-20 Your colleague looks at your data above and predicts that protein G will bind more strongly to the DNA at site A, compared to protein H.Which experiment above is critical for this prediction? A)#2 B)#3 C)#5 D)#6" class="answers-bank-image d-block" loading="lazy" > Figure 8-20 You develop a cell-free transcriptional system to study the effects of proteins G, H, and J on the transcription of gene Q.Using this system, you can examine the effects of adding these proteins to the transcriptional system in equal amounts and measuring how much gene Q is produced.When you add these proteins to the system, you get the results shown in Table 8-20. experiment reaulator protein added Q mRNA number 12345678 G −+−−++−+H−−+−+−++J−−−+−+++ made? no no yes yes no yes yes yes
Table 8-20 Your colleague looks at your data above and predicts that protein G will bind more strongly to the DNA at site A, compared to protein H.Which experiment above is critical for this prediction?