Question 11

(Multiple Choice)

You are interested in the regulation of gene Q.Proteins G, H, and J are proteins that are important for regulating gene Q, and bind to its promoter region in a sequence-specific fashion.Proteins G and H both bind to site "A" but cannot bind to site "A" at the same time.Protein J binds to site "B" on the promoter.The promoter region is diagrammed in Figure 8-20. You are interested in the regulation of gene Q.Proteins G, H, and J are proteins that are important for regulating gene Q, and bind to its promoter region in a sequence-specific fashion.Proteins G and H both bind to site A but cannot bind to site A at the same time.Protein J binds to site B on the promoter.The promoter region is diagrammed in Figure 8-20.   Figure 8-20 You develop a cell-free transcriptional system to study the effects of proteins G, H, and J on the transcription of gene Q.Using this system, you can examine the effects of adding these proteins to the transcriptional system in equal amounts and measuring how much gene Q is produced.When you add these proteins to the system, you get the results shown in Table 8-20.  <span class= experiment \text { experiment } reaulator protein added \text { reaulator protein added } Q mRNA \text { Q mRNA }  number  G HJ made? 1 no 2+ no 3+ yes 4+ yes 5++ no 6++ yes 7++ yes 8+++ yes \begin{array}{ccccc} \text { number } &\quad\quad\quad\text { G } & H & J & \quad\quad\text { made? } \\ \hline 1 &\quad\quad - & - & - & \text { no } \\ \hline 2 & \quad\quad+ & - & - & \text { no } \\ \hline 3 & \quad\quad- & + & - & \text { yes } \\ \hline 4 &\quad\quad - & - & + & \text { yes } \\ \hline 5 &\quad\quad + & + & - & \text { no } \\ \hline 6 &\quad\quad + & - & + & \text { yes } \\ \hline 7 & \quad\quad- & + & + & \text { yes } \\ \hline 8 & \quad\quad+ & + & + & \text { yes } \end{array} Table 8-20 Which proteins are likely to act as gene activators? A)G B)H C)J D)both H and J" class="answers-bank-image d-block" loading="lazy" > Figure 8-20
You develop a cell-free transcriptional system to study the effects of proteins G, H, and J on the transcription of gene Q.Using this system, you can examine the effects of adding these proteins to the transcriptional system in equal amounts and measuring how much gene Q is produced.When you add these proteins to the system, you get the results shown in Table 8-20.  experiment \text { experiment } reaulator protein added \text { reaulator protein added } Q mRNA \text { Q mRNA }
 number  G HJ made? 1 no 2+ no 3+ yes 4+ yes 5++ no 6++ yes 7++ yes 8+++ yes \begin{array}{ccccc}\text { number } &\quad\quad\quad\text { G } & H & J & \quad\quad\text { made? } \\\hline 1 &\quad\quad - & - & - & \text { no } \\\hline 2 & \quad\quad+ & - & - & \text { no } \\\hline 3 & \quad\quad- & + & - & \text { yes } \\\hline 4 &\quad\quad - & - & + & \text { yes } \\\hline 5 &\quad\quad + & + & - & \text { no } \\\hline 6 &\quad\quad + & - & + & \text { yes } \\\hline 7 & \quad\quad- & + & + & \text { yes } \\\hline 8 & \quad\quad+ & + & + & \text { yes }\end{array}

Table 8-20
Which proteins are likely to act as gene activators?


A)G
B)H
C)J
D)both H and J

Answer