Gene Amplification Can Be Detected By

Question

Gene amplification can be detected by
A)Observation of homogeneously staining regions using microscopy.
B)Deletion of the arm of a chromosome.
C)Observation of extrachromosomal "double minutes."
D)A and C.
E)None of the above.

Accepted Answer

Observation of homogeneously staining regions (HSRs) and extrachromosomal "double minutes" (DMs) are both indicative of gene amplification, with HSRs being localized amplification on a chromosome and DMs being extrachromosomal circles of DNA carrying amplified genes. Deletion of a chromosome arm is not indicative of gene amplification.