A Scorpion Primer

Question

A Scorpion primer
A) extends the DNA polymerase's range.
B) is directed at a specific DNA target sequence.
C) uses a single oligonucleotide to prime a specific sequence and to detect accumulated PCR product.
D) uses several oligonucleotides to prime sequences and acts as the beacon probe to detect hybrids.

Accepted Answer

Scorpion primers are designed to be a single oligonucleotide that both primes DNA synthesis for a specific sequence and acts as a probe to detect the PCR product, making them highly specific and efficient for real-time PCR applications.