The Steps for Performing the Northern Blot Test Include All

Question

The steps for performing the Northern blot test include all the following, except:
A) RNA is separated in an agarose gel.
B) The separated RNA is transferred from the agarose gel onto a membrane.
C) The membrane is then transferred to a gel so that the RNA can be immobilized in the gel.
D) The immobilized RNA is detected with a probe that hybridizes to the RNA species of interest.

Accepted Answer

The membrane is not transferred to a gel after it has been transferred from agarose gel, instead, it is directly probed with a labeled probe to detect the RNA species of interest.