The Steps for Performing the Southern Blot Test Include All

Question

The steps for performing the Southern blot test include all the following, except:
A) After the labeled probe is hybridized to the specific target, the DNA is electrophoresed again, then dried and read.
B) The DNA is first digested with restriction enzymes.
C) Once digested, the DNA is separated and immobilized onto a solid membrane.
D) Labeled probe is hybridized to the specific DNA sequence.

Accepted Answer

After the labeled probe is hybridized to the specific target, the DNA is not electrophoresed again, but rather subjected to autoradiography to visualize the labeled probe.