The Principle of Solid Support Hybridization (Often Called Blotting) Is

Question

The principle of solid support hybridization (often called blotting) is:
A) The target sequence is part of the liquid support, and the probe, which is attached to solid support, hybridizes to the target.
B) All the target nucleic acids are gathered in one spot on the electrophoresis gel. Then all the probes are flooded in the same area and produce a blot of duplexes.
C) The target sequence is part of the solid support, and the probe, which is in solution, hybridizes to the target.
D) The probe is electrophoresed in agar and then transferred to filter paper. The antibody-based probe is then applied to the filter paper, and blots appear where there is binding.

Accepted Answer

The principle of solid support hybridization, also called blotting, involves the target sequence being immobilized on a solid support (such as a nitrocellulose membrane). The probe, which is in solution, is then added and binds to the immobilized target sequence, forming a duplex. The hybridized probe can then be detected through various methods, such as chemiluminescence or fluorescence. This technique is commonly used in molecular biology to detect and analyze DNA or RNA molecules.