How is the Stability of a Given Hybrid Calculated

Question

How is the stability of a given hybrid calculated?
A) By calculating the number of certain types of bases in its structure
B) By calculating the length of the hybrid
C) By determining the melting temperature of a probe
D) By determining the ionic strength of the test solution

Accepted Answer

The stability of a given hybrid, particularly in the context of nucleic acid hybrids, is often determined by its melting temperature (Tm). The melting temperature is the temperature at which half of the hybrid molecules dissociate into single strands, and it provides a measure of the hybrid's stability under specific conditions.