Which of the Following Enzymes Cannot Catalyze the Formation of a Phosphodiester

Question

Which of the following enzymes cannot catalyze the formation of a phosphodiester bond?
A)endonuclease
B)RNA polymerase
C)DNA polymerase
D)ligase
E)none of the choices are correct.

Accepted Answer

Endonuclease enzymes catalyze the hydrolysis of phosphodiester bonds, but they do not catalyze the formation of phosphodiester bonds. RNA polymerase and DNA polymerase both catalyze the formation of phosphodiester bonds during transcription and DNA replication, respectively. Ligase enzymes also catalyze the formation of phosphodiester bonds, typically in the context of joining two separate strands of DNA or RNA. Therefore, A is the correct choice.