Which of the Following Enzymes Will Produce Blunt-Ended Cuts in DNA

Question

Which of the following enzymes will produce blunt-ended cuts in DNA?
A)EcoRI
B)HindIII
C)SmaI
D)BamHI
E)PstI

Accepted Answer

SmaI is a restriction enzyme that recognizes the sequence CCCGGG, which is a palindrome, and cleaves both strands of DNA at the same position leaving a blunt end. The other enzymes listed all produce sticky ends, where the ends of the DNA fragments have unpaired bases, allowing them to form hydrogen bonds with complementary bases on other DNA fragments.