Which of the Following is Not Used in a Sanger

Question

Which of the following is not used in a Sanger chain termination reaction?
A)ddATP
B)ddUTP
C)ddCTP
D)ddGTP
E)ddTTP

Accepted Answer

ddUTP is not used in a Sanger chain termination reaction. The other four choices (ddATP, ddCTP, ddGTP, and ddTTP) are used, each labeled with a different fluorescent dye, to terminate DNA synthesis at specific bases and generate a ladder of fragments with varying lengths that can be separated by size using gel electrophoresis.