Which of the Following is Used to Study the Interaction

Question

Which of the following is used to study the interaction of proteins with DNA?
A)S1 nuclease protection
B)DNase-1 footprinting
C)DNA fingerprinting
D)southern analysis
E)northern blotting

Accepted Answer

DNase-1 footprinting is used to study the interaction of proteins with DNA. It involves treating a DNA sample with DNase-1, which cleaves the DNA at random positions. However, if a protein is bound to the DNA at a specific site, it will protect that site from cleavage by the DNase-1. The resulting pattern of cleavage products can be visualized by gel electrophoresis and used to determine the binding site(s) of the protein on the DNA molecule.