Rapid Purification of Proteins Based on Size Can Best Be

Question

Rapid purification of proteins based on size can best be done using
A)gel filtration.
B)ion-exchange chromatography.
C)native gel electrophoresis.
D)2-D gel electrophoresis.
E)cation-exchange chromatography.

Accepted Answer

Gel filtration chromatography separates proteins based on their size or molecular weight. It is a type of size exclusion chromatography where larger molecules are excluded from the pores of the gel matrix and elute first, while smaller molecules enter the pores and elute later. This method is useful for the purification of proteins based on their size, as it allows for separation of closely related molecules that may differ in molecular weight.