Predict the Effect of the Addition of Excess Acetyltansferase to in Vitro

Question

Predict the effect of the addition of excess acetyltansferase to in vitro transcription assay.
A)It will likely loosen the interaction of histone with DNA.
B)It will lead to a tighter association of histone with DNA,resulting in reduced transcription.
C)It will have no effect on the interaction of DNA with histones.
D)There will be a complete repression of transcription.
E)None of the choices are correct.

Accepted Answer

Acetyltransferase adds acetyl groups to histones, which neutralizes their positive charge and decreases their affinity for the negatively charged DNA. This loosening of the interaction facilitates transcription by making the DNA more accessible to transcription machinery.