Temporal RGC Axons Exhibited Selectivity in the Stripe Assay When

Question

Temporal RGC axons exhibited selectivity in the stripe assay when:
A) posterior membranes were treated with PI-PLC.
B) posterior membranes were heat-inactivated.
C) anterior membranes were heat-inactivated.
D) purified ephrin-A5 was laid down in alternating stripes.
E) c and d

Accepted Answer

Selectivity was abolished when posterior membranes (but not anterior membranes) were heat-inactivated or treated with PI-PLC, implying that a GPI-linked repellant cue is present in posterior tectum. Purified ephrin-A5 mimics the repulsion.