Which Is/are the Correct Statement(s) About Confocal And/or Light-Sheet Microscopy

Question

Which is/are the correct statement(s) about confocal and/or light-sheet microscopy?
A) Confocal microscopy detects fluorescence from a single focal plane of tissue.
B) Confocal microscopy focuses light on a specific focal plane in a section of tissue.
C) Light-sheet microscopy detects fluorescence from a single focal plane of tissue.
D) Light-sheet microscopy uses a large objective lens to focus light on a large sheet of tissue.
E) Confocal microscopy is faster than light-sheet microscopy.
F) Both require fluorescent markers in the tissue.

Accepted Answer

AND F .
 Both confocal and light-sheet microscopy detect fluorescence so fluorescent markers are required. Confocal microscopy focuses light on a specific focal plane and scans across the plane to collect an image. Light-sheet microscopy illuminates a thin sheet of the specimen from the side.