How Can Single, Identifiable Neurons Be Labeled? Choose All That

Question

How can single, identifiable neurons be labeled? Choose all that apply.
A) Intracellular dye fill
B) Using a thy1-promotor to drive GFP expression
C) Anterograde tracers
D) Retrograde traces
E) Golgi stain
F) Nissl stain

Accepted Answer

AND E
Intracellular recordings are made from single cells and dyes can be injected into cells during recordings. It is more difficult to drive GFP expression in single neurons with GFP, but the thy1 gene is preferentially expressed in excitatory neurons and can label a subset of neurons due to random integration of the transgene. Golgi stains subsets of neurons, although it can be difficult to distinguish individual neurons. Anterograde and retrograde tracers label many neurons and so it is difficult to dissociate single neurons. Nissl stains all neurons and glia.