Southern Blotting and Hybridization is a Technique That Uses Specific

Question

Southern blotting and hybridization is a technique that uses specific probes to analyze:
A) restriction fragment length polymorphisms.
B) complex mixtures of any nucleic acid.
C) centromeres or telomeres on condensed chromosomes.
D) DNA fingerprints.
E) both A and D.

Accepted Answer

Southern blotting and hybridization is commonly used to analyze restriction fragment length polymorphisms, which are variations in the lengths of DNA fragments resulting from different sequences or mutations. This technique is also used to create DNA fingerprints, which can be used for genetic identification. Therefore, choices A and D are both correct. The other choices, such as complex nucleic acid mixtures or centromeres/telomeres, are not typically analyzed using Southern blotting and hybridization.