After Promoter Clearance by RNA Polymerase II

Question

After promoter clearance by RNA polymerase II:
A) all associated factors remain bound to the polymerase for elongation.
B) a subset of factors remain at the promoter region.
C) no further elongation factors are acquired by the polymerase.
D) no further chromatin remodeling is required.
E) transcription continues until the polymerase reaches the poly (A) tail.

Accepted Answer

After promoter clearance, a subset of transcription factors, such as the TFIID complex, remain at the promoter region to facilitate subsequent rounds of transcription initiation. However, most elongation factors are recruited to the polymerase during the process of mRNA synthesis.

Answer: C
 Elongation factors, such as the P-TEFb complex, are recruited to the polymerase during the process of mRNA synthesis and are not present at the promoter region.

Answer: D
 Chromatin remodeling may still be required during the elongation phase to facilitate the movement of the polymerase through the nucleosomes.

Answer: E
 Transcription may terminate before the polymerase reaches the poly(A) tail, depending on the presence of termination signals and other factors.