Question 3

(Multiple Choice)

How is a fluorescent tag that is unique for each nucleotide incorporated into the newly synthesized DNA using the chain termination protocol for DNA sequencing?


A) Each of the ddNTPs is labeled with a specific fluorophore
B) The primer strand is labeled with a specific fluorophore
C) The template strand is labeled prior to the experiment with complimentary fluorophores
D) The 5'-end is labeled with a fluorophore.
E) All dNTPs are labeled with a fluorophore

Answer

A