In the hydrolysis of p-nitrophenylacetate by chymotrypsin all of the following are correct EXCEPT:
A) the first product is acetic acid. B) an acetyl-enzyme intermediate forms during the mechanism. C) attack of a water molecule on the acyl-enzyme intermediate yields the second product. D) the active site contains a serine residue, an unprotonated histidine, and an unprotonated aspartate residue at pH 7.0 prior to binding the substrate. E) none of the above.