An A260/A280 Ratio of Less Than 1.8 Indicatesـــــــــــــــــــــــــ

Question

An A260/A280 ratio of less than 1.8 indicatesـــــــــــــــــــــــــ .
A)pure DNA
B)pure RNA
C)protein contamination of DNA
D)RNA contamination of DNA

Accepted Answer

The A260/A280 ratio is used to assess the purity of DNA and RNA samples, with a ratio of ~1.8 indicating pure DNA and ~2.0 indicating pure RNA. A ratio less than 1.8 suggests protein contamination in DNA samples, as proteins absorb strongly at 280 nm.