Which of the following is the most sensible order of techniques for mapping a chromosome and then locating a specific disease gene?

A)contig alignment;restriction mapping;RFLP linkage analysis;chromosomal walking
B)chromosomal walking;contig alignment;restriction mapping;RFLP linkage analysis
C)restriction mapping;chromosomal walking;contig alignment;RFLP linkage analysis
D)chromosomal walking;restriction mapping;RFLP linkage analysis;contig alignment
E)restriction mapping;contig alignment;RFLP linkage analysis;chromosomal walking