Which of the Following Is/are TRUE About Pyrosequencing

Question

Which of the following is/are TRUE about pyrosequencing?
A)Pyrosequencing uses ddNTPs as substrates.
B)Pyrosequencing uses DNA polymerase and two other enzymes,sulfurylase and luciferase,in the reaction.
C)PPi is released for every nucleotide incorporated into the growing strand.
D)All of the answer options are correct.
E)Pyrosequencing uses DNA polymerase,sulfurylase,and luciferase,and PPi is released for every nucleotide incorporated into the growing strand.

Accepted Answer

Pyrosequencing uses DNA polymerase, sulfurylase, and luciferase in the reaction, and PPi is released for every nucleotide incorporated into the growing strand. Therefore, option E is the correct answer as it includes all the true statements about pyrosequencing.