Identification of MRNA and Initiation of Priming for CDNA Synthesis

Question

Identification of mRNA and initiation of priming for cDNA synthesis is accomplished by:
A)purifying only cytosolic RNAs before initiating the process.
B)coupling cDNA synthesis to exon splicing.
C)detecting the 5′ cap sequence to initiation cDNA synthesis.
D)use of oligo-dT to prime cDNA synthesis from the polyA tail.
E)column purification of mRNA sequences.

Accepted Answer

The use of oligo-dT to prime cDNA synthesis from the polyA tail is a common method for identifying mRNA and initiating cDNA synthesis. The polyA tail is found at the 3' end of most eukaryotic mRNA, and is composed of a string of adenine nucleotides that can be specifically targeted by oligo-dT primers. This allows for selective priming of cDNA synthesis from mRNA templates, enabling specific identification and quantification of mRNA transcripts.