Recombinant DNA Techniques Typically Require the Action Of

Question

Recombinant DNA techniques typically require the action of:
A)DNA polymerase and phosphatase.
B)restriction enzymes and DNA ligase.
C)RNA polymerase and RNA primase.
D)reverse transcriptase.
E)DNA helicase.

Accepted Answer

Recombinant DNA techniques often involve cutting DNA with restriction enzymes and then joining different pieces of DNA together using DNA ligase. This allows for the creation of new DNA sequences with desired traits. DNA polymerase and phosphatase are involved in DNA sequencing and analysis, but not typically in recombinant DNA techniques. RNA polymerase and RNA primase are involved in transcription and not typically used in recombinant DNA techniques. Reverse transcriptase is used in synthesizing cDNA from RNA, but not typically involved in recombinant DNA techniques. DNA helicase is involved in DNA replication and repair but not typically used in recombinant DNA techniques.