In Recombinant DNA Technology,DNA is Most Often Cut Using

Question

In recombinant DNA technology,DNA is most often cut using:
A)DNA ligase.
B)DNA polymerase.
C)DNA gyrase.
D)restriction endonucleases.
E)terminal transferase.

Accepted Answer

In recombinant DNA technology, DNA is most often cut using restriction endonucleases. These enzymes recognize and cut specific nucleotide sequences in DNA, allowing for the precise manipulation of DNA fragments in the laboratory. DNA ligase is used to seal the ends of DNA fragments together after they have been cut with restriction endonucleases. DNA polymerase is an enzyme that synthesizes DNA and is not typically used for cutting DNA in recombinant DNA technology. DNA gyrase is a type of topoisomerase that helps to relieve tension in twisted DNA strands and is not typically involved in DNA cutting. Terminal transferase is a DNA polymerase that adds nucleotides to the end of a DNA strand and is not typically used for cutting DNA in recombinant DNA technology.