The -10 and -35 Boxes Found in Most Bacterial Promoter

Question

The -10 and -35 boxes found in most bacterial promoter elements were discovered by:
A)comparing the DNA sequences in front of highly expressed genes and determining nucleic acids that were common (consensus).
B)the finding that RNA polymerase begins translation when a 10-nucleotide followed by a 35-nucleotide repeat of guanine ("G")are encountered.
C)comparing bacterial promoters to known eukaryotic promoters.
D)examining data from the human genome project.
E)Robert Koch as he characterized the microbe that causes tuberculosis.

Accepted Answer

The -10 and -35 boxes were discovered by comparing the DNA sequences in front of highly expressed genes and determining nucleic acids that were common (consensus). This allowed for the identification of a consensus sequence for bacterial promoter elements.