Question 42

(Essay)

Draw all of the DNA fragments that would result after 3 rounds of PCR if you start with: linear template DNA that is 2kb in length,two primers each of which have complementary sequences located 0.5 kb in from each end of the template and which are properly oriented for PCR,and all other components necessary for PCR.Label all DNA ends with regards to polarity and give fragment sizes where applicable.

Answer