A Physical Map of a Chromosome Can Be Created By

Question

A physical map of a chromosome can be created by:
A) Mapping the restriction cleavage sites in the chromosome
B) Using electron microscopy
C) Using western blotting
D) Using recombination experiments
E) All of these

Accepted Answer

Mapping the restriction cleavage sites in the chromosome allows for the identification and location of specific DNA sequences on the chromosome, which can then be used to create a physical map. Electron microscopy can be used to visualize the chromosome, but does not provide specific location information. Western blotting is a technique used to detect specific proteins and would not be useful in creating a physical map of a chromosome. Recombination experiments can provide information on the relative location of genes on a chromosome, but may not give a complete picture of the physical structure of the chromosome.