Site Specific Mutagenesis is Commonly and Efficiently Performed by Which

Question

Site specific mutagenesis is commonly and efficiently performed by which procedure?
A) PCR
B) Southern Blotting
C) Nothern Blotting
D) Western Blotting
E) Complementation Testing

Accepted Answer

PCR can be used to create site-specific mutations in DNA by designing primers with the desired mutation. The PCR product can then be used directly for cloning or further manipulation. Southern, Northern, and Western blotting are techniques used for detecting specific molecules such as DNA, RNA, or protein, respectively. Complementation testing is not a method for site-specific mutagenesis, but rather a genetic test used to determine if two mutations are in the same gene or different genes.