Which of the Following is a Method by Which a Gene

Question

Which of the following is a method by which a gene can be isolated from a DNA library?
A) In situ colony hybridization
B) Genetic complementation
C) Genetic selection
D) In situ plaque hybridization
E) All of these

Accepted Answer

All of the listed options are methods by which a gene can be isolated from a DNA library. 
- In situ colony hybridization and in situ plaque hybridization involve identifying a colony or plaque containing the desired gene through hybridization with a specific probe. 
- Genetic complementation involves introducing the library into a mutant cell or organism in which the gene of interest is non-functional, and identifying the library clone that restores function. 
- Genetic selection involves selecting for library clones that exhibit a specific phenotype of interest.