Which of the Following is a Disadvantage of the PCR

Question

Which of the following is a disadvantage of the PCR process?
A) Fragments longer than 35kb cannot be efficiently amplified
B) It replicates double stranded DNA exponentially
C) It requires DNA to be denatured
D) All of these
E) None of these

Accepted Answer

PCR (Polymerase Chain Reaction) is limited in its ability to amplify very long fragments of DNA efficiently. Typically, it is most effective for amplifying fragments up to about 10kb in length, although with specialized techniques, fragments up to about 35kb can sometimes be amplified. Beyond this range, efficiency drops significantly.