Which of the Following is the Correct Order of Steps

Question

Which of the following is the correct order of steps in PCR?
A) Extension of DNA, denaturation of DNA, annealing of primers
B) Denaturation of DNA, extension of DNA, annealing of primers
C) Denaturation of DNA, annealing of primers, extension of DNA
D) Annealing of primers, extension of DNA, denaturation of DNA
E) Annealing of primers, denaturation of DNA, extension of DNA

Accepted Answer

The correct order of steps in PCR is denaturation of DNA, annealing of primers, and extension of DNA. This is because denaturation (separation of DNA strands) is needed to expose the target DNA region, annealing of primers is needed to bind the primers specifically to the target DNA region, and extension (synthesis of new DNA strand using the primers as templates) is needed to amplify the target DNA region.