Which of the Following is an Essential Component in a Cloning

Question

Which of the following is an essential component in a cloning vector?
A) Origin of replication
B) Selectable marker gene
C) Unique restriction enzyme site
D) Origin of replication and Unique restriction enzyme site
E) All of these

Accepted Answer

All of these components are essential in a cloning vector. The origin of replication ensures that the vector can replicate in the host cell, the selectable marker gene allows for selection and identification of cells with the vector, and the unique restriction enzyme site allows for insertion of the target DNA fragment into the vector.