Polypeptide chain elongation and termination of translation differ in prokaryotes and eukaryotes in which of the following ways?

A) Binding of the aminoacyl-tRNA to the A-site
B) Number of release factors
C) Transfer of the growing peptide from the tRNA in the P-site to the tRNA in the A-site
D) Translocation requirement of GTP hydrolysis
E) Translocation of the ribosome along the mRNA to position the next codon in the A-site