Eukaryotes Were Found to Have Multiple Replicons Per Chromosome By

Question

Eukaryotes were found to have multiple replicons per chromosome by:
A) ²H thymidine pulse experiments
B) ³H thymidine pulse-chase experiments
C) ¹H thymidine pulse experiments
D) ²H thymidine pulse-chase experiments
E) None of these

Accepted Answer

³H thymidine pulse-chase experiments were used to demonstrate that eukaryotic chromosomes contain multiple origins of replication, which are activated at different times during the S phase of the cell cycle. This method involves labeling newly synthesized DNA with tritiated thymidine (³H thymidine) and then following the distribution of the label over time.