For the Polymerase Chain Reaction to Occur,____

Question

For the polymerase chain reaction to occur,____.  
A) the reaction temperature must be cycled from low to high
B) all DNA fragments must be identical
C) the DNA must remain double stranded
D) a sticky end must be available for the ligase enzyme to function
E) a large sample of DNA is needed

Accepted Answer

The polymerase chain reaction (PCR) requires a cycle of high and low temperatures to denature, anneal, and extend the DNA fragments. This is known as thermal cycling.