In Step 2 (Above)in the Identification of the Colony That

Question

In step 2 (above)in the identification of the colony that contains a targeted gene,____.  
A) bacterial cells are cloned
B) bacterial colonies are transferred
C) bacterial cells are ruptured
D) DNA is lysed
E) cells with the targeted gene are identified

Accepted Answer

In step 2, bacterial colonies containing the targeted gene are transferred from the original plate onto a new plate containing agar media. This is done to isolate a pure colony for further analysis. Therefore, the correct choice is B. Option A is incorrect because bacterial cells are cloned in previous steps. Option C is incorrect because bacterial cells are not ruptured in this step. Option D is incorrect because DNA lysis is not required in this step. Option E is incorrect because cells with the targeted gene are not identified in this step.