In Panel 4,the Base-Paired DNA Fragments are Joined Together with ____

Question

In panel 4,the base-paired DNA fragments are joined together with ____.  
A) DNA polymerase
B) RNA polymerase
C) reverse transcriptase
D) DNA ligase
E) restriction endonuclease

Accepted Answer

The base-paired DNA fragments in panel 4 are joined together with DNA ligase. DNA ligase is an enzyme that catalyzes the formation of a phosphodiester bond between adjacent nucleotides, sealing the nicks in the sugar-phosphate backbone of DNA strands. This reaction is essential for the formation of recombinant DNA, as it allows the insertion of foreign DNA fragments into a vector backbone. DNA polymerase, RNA polymerase, reverse transcriptase, and restriction endonuclease do not have the ability to join DNA fragments together in this fashion.