Which of the Following is a Serious Concern When Performing

Question

Which of the following is a serious concern when performing PCR?
A)The high temperature needed may denature the DNA.
B)Restriction enzymes are difficult to obtain in adequate quantities.
C)Contaminating DNA may be introduced and amplified.
D)Lab personnel may be exposed to radiation.
E)PCR is a very time-consuming process.

Accepted Answer

Contaminating DNA may be inadvertently introduced during PCR and can be amplified along with the target DNA, leading to false results or erroneous conclusions. It is crucial to maintain strict aseptic techniques and use appropriate controls to detect contamination. The other options are not generally applicable concerns when performing PCR.