In Order to Ensure Compatibility Between the Target DNA and the Plasmid

Question

In order to ensure compatibility between the target DNA and the plasmid DNA,
A)target DNA is removed from cells and isolated.
B)cloning host is treated with calcium chloride and receives plasmid.
C)target DNA and plasmid are treated with the same restriction endonuclease.
D)desired protein is produced by cloning host.
E)gene is amplified by multiplication of cloning host.

Accepted Answer

The best choice to ensure compatibility between the target DNA and the plasmid DNA is to treat them with the same restriction endonuclease. This creates compatible ends on both the target DNA and plasmid, allowing them to join together via ligation.